Coding

Part:BBa_K1556000:Design

Designed by: Kenny Kostenbader   Group: iGEM14_Rutgers   (2014-10-15)


Murine Terminal Transferase


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 268
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 268
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 446
    Illegal BglII site found at 1299
    Illegal BglII site found at 1538
    Illegal BamHI site found at 3
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 268
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 268
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 143
    Illegal SapI.rc site found at 1069
    Illegal SapI.rc site found at 1254


Design Notes

This is a eukaryotic sequence (the arginine codon is fairly rare in E. coli - protein production is low).


Source

Sourced from a plasmid from Hardvard Med School's plasmid-ID repository.

References